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The time-course of DNA repair after gamma irradiation was measured in HeLa cells at various temperatures. Unscheduled DNA synthesis was estimated by incorporation of 3H-thymidine in presence of hydroxyurea. To detect the ligase reaction, the number of single strand breaks (SSB) was determined by centrifugation in alcaline sucrose as well as by hydroxylapatite chromatography after partial denaturation. In addition, the temperature dependence of DNA polymerase and DNase reaction in cell-free systems were measured. These data were compared with the reduction of colony-forming ability of the cells caused by gamma irradiation and following repair at various temperatures. All steps of repair proceed faster at 41--43 degrees than at 37 degrees but cells are most resistant to gamma irradiation at 37 degrees. We therefore assume that the DNA repair process at 42 degrees is faster but more error prone than at 37 degrees.
P Weniger, and
E Wawra, and
I Dolejs
May 1978,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
P Weniger, and
E Wawra, and
I Dolejs
January 1974,
Postepy biochemii,
P Weniger, and
E Wawra, and
I Dolejs
December 1976,
Zeitschrift fur Krebsforschung und klinische Onkologie. Cancer research and clinical oncology,
P Weniger, and
E Wawra, and
I Dolejs
January 1981,
Bulletin du cancer,
P Weniger, and
E Wawra, and
I Dolejs
December 1980,
Bratislavske lekarske listy,
P Weniger, and
E Wawra, and
I Dolejs
July 1975,
Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology,
P Weniger, and
E Wawra, and
I Dolejs
October 1975,
Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology,
P Weniger, and
E Wawra, and
I Dolejs
January 1978,
Postepy biochemii,
P Weniger, and
E Wawra, and
I Dolejs
August 1974,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
P Weniger, and
E Wawra, and
I Dolejs
August 1974,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
