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We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and the glucose oxidase oxygen-rate method (y = 0.99x + 14; r = 0.99) with the Beckman Glucose Analyzer. Sample-to-sample interaction was less than 1%. Ascorbic acid or uric acid in concentrations as high as 200 mg/L were without demonstrable effect on results for glucose. Intra- and inter-assay precisions (CV) were 1.6 and 2.3%, respectively. The upper limit of linearity was about 5 g/L. Adaptation of the Trinder method for glucose to the SMAC is simple and provides an analytically acceptable and economical alternative to the methods ordinarily used with the SMAC.
G V Purcell, and
D B Behenna, and
P R Walsh
July 1978,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
October 1973,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
May 1974,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
February 1973,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
November 1988,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
February 1976,
Clinical chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
January 1981,
The Journal of automatic chemistry,
G V Purcell, and
D B Behenna, and
P R Walsh
September 2006,
Die Pharmazie,
G V Purcell, and
D B Behenna, and
P R Walsh
October 1976,
The American journal of medical technology,
G V Purcell, and
D B Behenna, and
P R Walsh
October 1977,
Clinical chemistry,
