Biomaterial Database

Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig. 1974

B R Eisenberg, and A M Kuda, and J B Peter

A quantitative analysis of the volumes, surface areas, and dimensions of the ultrastructural components in the soleus muscle fibers of the guinea pig was made by using point counting methods of stereology. Muscle fibers have structural orientation (anisotropy) and have spatial gradients of the structures within the fiber; therefore the standard stereological methods were modified where necessary. The entire analysis was repeated at two section orientations to test the modifications and identical results obtained from both. The volume of lipid droplets was 0.20 +/- 0.06% (mean +/- standard error, n = 5 animals) and the nuclei volume was 0.86 +/- 0.20% of the fiber volume. The total mitochondrial volume was 4.85 +/- 0.66% of the fiber volume with about one-third being found in an annulus within 1 microm of the sarcolemma. The mitochondrial volume in the remaining core of the fiber was 3.6 +/- 0.4%. The T system has a volume of 0.14 +/- 0.01% and a surface area of 0.064 +/- 0.005 microm(2)/microm(3) of the fiber volume. The surface area of the sarcolemma is 0.116 +/- 0.013 microm(2)/microm(3) which is twice the T system surface area. The volume of the entire sarcoplasmic reticulum is 3.52 +/- 0.33% and the surface area is 0.97 +/- 0.09 microm(2)/microm(3). The sarcoplasmic reticulum is composed of the terminal cisternae whose volume is 1.04 +/- 0.19% and surface area is 0.24 +/- 0.05 microm(2)/microm(3). The tubules of the sarcoplasmic reticulum in the I band and A band have volumes of 1.97 +/- 0.24% and 0.51 +/- 0.08%, and the surface areas of the I and A band reticulum are 0.56 +/- 0.07 microm(2)/microm(3) and 0.16 +/- 0.04 microm(2)/microm(3), respectively. The Z line width, myofibril and fiber diameters were measured.

UI	MeSH Term	Description	Entries
D008055	Lipids	A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)	Lipid
D008297	Male		Males
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008931	Mitochondria, Muscle	Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available.	Sarcosomes,Mitochondrion, Muscle,Muscle Mitochondria,Muscle Mitochondrion,Sarcosome
D008968	Molecular Conformation	The characteristic three-dimensional shape of a molecule.	Molecular Configuration,3D Molecular Structure,Configuration, Molecular,Molecular Structure, Three Dimensional,Three Dimensional Molecular Structure,3D Molecular Structures,Configurations, Molecular,Conformation, Molecular,Conformations, Molecular,Molecular Configurations,Molecular Conformations,Molecular Structure, 3D,Molecular Structures, 3D,Structure, 3D Molecular,Structures, 3D Molecular
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006614	Hindlimb	Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)	Hindlimbs