Biomaterial Database

[Importance of the sialic acid moiety for the heterogeneity in human fibrinogen]. 1979

C Kuyas, and A Haeberli, and P W Straub

To determine whether the observed heterogeneity of gamma- and B beta-polypeptide chains of human fibrinogen (2 and 1 sialic acid residue per chain) is due to differences in sialic acid content, fibrinogen was desialatgen was compared with normal fibrinogen. The gamma-chain heterogeneity observed in normal fibrinogen was absent in asialofibrinogen, whereas the B beta-chain heterogeneity appeared to be unaffected. Although the variants were indistinguishable on SDS-PAGE, isoelectric focusing in the presence of urea demonstrated heterogeneities of both gamma- and B beta-cahins even in asialofibrinogen. However, fewer bands were recognized in asialofibrinogen. The difference in sialic acid content of the gamma- and B beta-chain variants of human fibrinogen therefore explains on part of the polypeptide chain heterogeneity.

UI	MeSH Term	Description	Entries
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D002852	Chromatography, Ion Exchange	Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.	Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D005340	Fibrinogen	Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.	Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012794	Sialic Acids	A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.	N-Acetylneuraminic Acids,Acids, N-Acetylneuraminic,Acids, Sialic,N Acetylneuraminic Acids