Automated radioimmunoassay of nicotine. 1979

A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman

We have developed an automated nonequilibrium procedure for the radioimmunoassay of nicotine. The use of a unique iodinated nicotine derivative in this procedure gave a sensitivity of 10 micrograms/l for nicotine with a between-run precision of 7.4% and within-run precision of 6.0%. Nicotine levels of 60 to 67 micrograms/ml were found in subjects 15 min after smoking one standard cigarette. The technique herein reported is a very rapid, and sensitive radioimmunoassay for nicotine and facilitates the determination of nicotine in smoking subjects during the actual process of smoking.

UI MeSH Term Description Entries
D008297 Male Males
D009538 Nicotine Nicotine is highly toxic alkaloid. It is the prototypical agonist at nicotinic cholinergic receptors where it dramatically stimulates neurons and ultimately blocks synaptic transmission. Nicotine is also important medically because of its presence in tobacco smoke. Nicotine Bitartrate,Nicotine Tartrate
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D012907 Smoking Willful or deliberate act of inhaling and exhaling SMOKE from burning substances or agents held by hand. Smoking Behaviors,Smoking Habit,Behavior, Smoking,Behaviors, Smoking,Habit, Smoking,Habits, Smoking,Smoking Behavior,Smoking Habits

Related Publications

A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
May 1975, Biochemical and biophysical research communications,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
January 1987, IARC scientific publications,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
January 1993, IARC scientific publications,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
September 1975, Laboratory practice,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
November 1977, Analytical chemistry,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
January 1983, Zeitschrift fur medizinische Laboratoriumsdiagnostik,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
April 1978, Medical laboratory sciences,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
October 1976, Science (New York, N.Y.),
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
October 1976, Clinical chemistry,
A Castro, and N Monji, and H Malkus, and W Eisenhart, and H McKennis, and E R Bowman
January 1982, Methods in enzymology,
Copied contents to your clipboard!