Biomaterial Database

Changes in glycoproteins of liver plasma membranes from rats treated with D-galactosamine. 1979

F Bertrand, and D Veissiere, and J Picard

D-Galactosamine administration to rats (400 mg/kg) by intraperitoneal injection induced biochemical alterations in liver plasma membranes. Alterations were studied 4, 16 and 24 h after D-galactosamine injection. Plasma membrane 5'-mononucleotidase activity decreased to 40% of control values. Carbohydrate composition was significantly changed. After 24 h D-galactosamine administration, the diminution in plasma membrane sialic acids and hexoses reached 30% of control values. As detected by SDS-acrylamide gel electrophoresis, high molecular weight glycoproteins of D-galactosamine-treated plasma membranes were modified. Moreover, the incorporation of [35S]-sulfate into membrane glycoproteins decreased after D-galactosamine administration (40--60% of control). The present results show that biochemical alterations in rat liver plasma membranes appear soon after D-galactosamine injection. Marked changes are observed in cell surface glycoproteins, especially in sialoglycoproteins and sulfated glycoproteins.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D009708	Nucleotidases	A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005688	Galactosamine
D005944	Glucosamine		2-Amino-2-Deoxyglucose,Dona,Dona S,Glucosamine Sulfate,Hespercorbin,Xicil,2 Amino 2 Deoxyglucose,Sulfate, Glucosamine
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013431	Sulfates	Inorganic salts of sulfuric acid.	Sulfate,Sulfates, Inorganic,Inorganic Sulfates