Effect of incubation temperature on T-agglutination typing of Streptococcus pyogenes. 1969

J F Padula, and R R Facklam, and M D Moody

The temperature of incubation affected the typability of beta-hemolytic group A streptococci by T-agglutination tests. When strains could not be typed after routine incubation at 30 C, they were incubated at 22 to 25 C, and nearly a 10% increase in typability was achieved. The clinical source of the strains was related to their typability. Incubation at the lower temperature was required for successful typing of higher percentages of strains from the skin and other clinical sources than from the throat. Sixty per cent of the skin strains were represented by six serotypes. Of these, 53% of the strains required incubation at 22 to 25 C before they could be typed.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D010612 Pharyngitis Inflammation of the throat (PHARYNX). Sore Throat,Pharyngitides,Sore Throats,Throat, Sore
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D012874 Skin Diseases, Infectious Skin diseases caused by bacteria, fungi, parasites, or viruses. Infectious Skin Diseases,Disease, Infectious Skin,Diseases, Infectious Skin,Infectious Skin Disease,Skin Disease, Infectious
D013290 Streptococcal Infections Infections with bacteria of the genus STREPTOCOCCUS. Group A Strep Infection,Group A Streptococcal Infection,Group A Streptococcal Infections,Group B Strep Infection,Group B Streptococcal Infection,Group B Streptococcal Infections,Infections, Streptococcal,Infection, Streptococcal,Streptococcal Infection
D013297 Streptococcus pyogenes A species of gram-positive, coccoid bacteria isolated from skin lesions, blood, inflammatory exudates, and the upper respiratory tract of humans. It is a group A hemolytic Streptococcus that can cause SCARLET FEVER and RHEUMATIC FEVER. Flesh-Eating Bacteria,Streptococcus Group A,Bacteria, Flesh-Eating
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D014946 Wound Infection Invasion of a wound by pathogenic microorganisms. Infection, Wound,Infections, Wound,Wound Infections

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