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We have developed a system for the in vitro transcription of specific genes in rooster liver chromatin by endogenous RNA polymerase II that maintains the specificity of transcription in vivo. Radioactive transcripts synthesized in vitro were identified and quantitated by hybridization to a vast excess of cloned cDNA. The cDNA preparations employed corresponded to vitellogenin mRNA, the synthesis of which is responsive to estrogen stimulation in vivo, and chicken serum albumin mRNA, the synthesis of which is not significantly affected by estrogen stimulation in vivo. Comparing the pattern of transcription of the albumin and vitellogenin genes in chromatin from the liver of the normal rooster with the pattern in chromatin from the liver of the estrogen-stimulated rooster, we found that prior estrogen treatment of the rooster is attended by a slight decrease in the differential rate of transcription of the albumin gene and approximately a 10-fold increase in the differential rate of transcription of the vitellogenin gene. Because this pattern of transcription reflects the estrogen-induced changes in transcription observed in vivo, chromatin preparations from the livers of normal and estrogen-stimulated roosters can be used to investigate regulation of specific gene transcription at the molecular level in vitro.
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
July 1985,
Nucleic acids research,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
April 2022,
Biochemical and biophysical research communications,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
September 1974,
Cell,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
January 2023,
Molecular and cellular biology,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
June 1993,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
April 1973,
Biochemical and biophysical research communications,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
October 1973,
Journal of molecular biology,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
March 2008,
American journal of physiology. Renal physiology,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
June 1990,
Current opinion in cell biology,
K P Mullinix, and
M B Meyers, and
J L Christmann, and
R G Deeley, and
J I Gordon, and
R F Goldberger
April 2020,
Science advances,
