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We have recently found that aphidicolin, a tetracyclic diterpene-tetraol produced by several fungi, blocks DNA synthesis of sea urchin embryos by interfering with the activity of DNA polyermase alpha. These cells fail to proliferate in the presence of aphidicolin. In continuation of these studies, we determined the drug-sensitive stage in the first cell cycle of the sea urchin Clypeaster japonicus embryo. In continuous exposure to aphidicolin (2 micrograms/ml) from five minutes after fertilization, mitotic division of the embryo was completely suppressed. Embryos were exposed to the drug at progressively later intervals and their capability for cytokinesis was examined. Evidence was thereby obtained that aphidicolin acts at the S-period to inhibit DNA synthesis resulting in developmental arrest of the embryo.
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
June 1985,
Biokhimiia (Moscow, Russia),
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
August 1985,
The Journal of experimental zoology,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
January 1985,
Development, growth & differentiation,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
November 1976,
Developmental biology,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
January 1979,
Development, growth & differentiation,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
June 1990,
Development, growth & differentiation,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
October 1987,
Development, growth & differentiation,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
November 1983,
Molecular pharmacology,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
May 1987,
Molecular pharmacology,
S Ikegami, and
S Amemiya, and
M Oguro, and
H Nagano, and
Y Mano
September 1981,
Experimental cell research,
