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It has been reported that inactive (acid-activable) human renin could be converted into the active form by adding urinary kallikrein to acid-pretreated plasma. Without prior acidification, however, only a small portion of the total amount of inactive renin present in plasma was converted (activated) by kallikrein, probably because native plasma contains protease (kallikrein) inhibitors that are destroyed by acid. We have separated inactive and active renin by DEAE-Sepharose column chromatography of normal human plasma at pH 7.5 and a linearly increasing sodium gradient. Inactive renin isolated in this way could be activated at pH 7.5 by highly purified pancreas and urinary kallikreins. With the semipurified preparation of inactive renin, prior acidification was not required for obtaining virtually complete activation by kallikrein. The kallikreins were effective at concentrations as low as 1 x 10(-8) mol/liter. It is therefore possible that one or more tissue kallikreins act as physiological activators of inactive renin.
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
August 1979,
Biochimica et biophysica acta,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
October 1982,
The Journal of clinical endocrinology and metabolism,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
January 1987,
Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
January 1980,
Clinical and experimental pharmacology & physiology,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
November 1983,
Horumon to rinsho. Clinical endocrinology,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
August 1983,
Ugeskrift for laeger,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
January 1982,
Hypertension (Dallas, Tex. : 1979),
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
January 1982,
Clinical and experimental hypertension. Part A, Theory and practice,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
January 1981,
Biochimica et biophysica acta,
F H Derkx, and
H L Tan-Tjiong, and
A J Man in't Veld, and
M P Schalekamp, and
M A Schalekamp
October 1983,
Japanese circulation journal,
