BIOMAT Database Logo BIOMAT Database Logo

Measurement of arachidonic acid in the plasma by gas-liquid chromatography-flame ionization using dihomo-gamma-linolenic acid as an internal standard. 1979

J G Gerber, and J S Barnes, and A S Nies

A gas-liquid chromatography-flame ionization method is described for measuring arachidonic acid in plasma using dihomo-gamma-linolenic acid as an internal standard. We found this technique to -e reproducible, and quicker and superior to previously described techniques because of the similar physico-chemical properties of the unsaturated fatty acid internal standard and arachidonic acid. In addition, we observed that the use of the saturated fatty acid, n-tricosanoic acid, was unsatisfactory as an internal standard because of its poor extractability from plasma as compared to arachidonic acid.

UI MeSH Term Description Entries
D002849 Chromatography, Gas Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001095 Arachidonic Acids Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic
D015126 8,11,14-Eicosatrienoic Acid A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5. Homo-gamma Linolenic Acid,8,11,14 Eicosatrienoic Acid,Dihomo-gamma-Linolenic Acid,Dihomogammalinolenic Acid,Ro 12-1989,Dihomo gamma Linolenic Acid,Homo gamma Linolenic Acid,Linolenic Acid, Homo-gamma,Ro 12 1989,Ro 121989

Related Publications

J G Gerber, and J S Barnes, and A S Nies
Measurement of chloroprocaine and procaine in plasma by flame ionization gas-liquid chromatography.
September 1978, Clinical chemistry,
J G Gerber, and J S Barnes, and A S Nies
Determination of valproic acid in human plasma using dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection.
October 2015, Iranian journal of basic medical sciences,
J G Gerber, and J S Barnes, and A S Nies
Determination of bromazepam in plasma with an internal standard by gas-liquid chromatography.
July 1975, Arzneimittel-Forschung,
J G Gerber, and J S Barnes, and A S Nies
Quantitative measurement of amphetamine in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry using (2H5)amphetamine as internal standard.
October 1994, Biological mass spectrometry,
J G Gerber, and J S Barnes, and A S Nies
Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard.
March 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
J G Gerber, and J S Barnes, and A S Nies
Metabolism of arachidonic acid by platelets: utilization of arachidonic acid by human platelets in presence of linoleic and dihomo-gamma-linolenic acids.
December 1978, Zeitschrift fur Ernahrungswissenschaft,
J G Gerber, and J S Barnes, and A S Nies
Modification of genetic damage by dihomo-gamma-linolenic acid.
March 1994, Bulletin of environmental contamination and toxicology,
J G Gerber, and J S Barnes, and A S Nies
Determination of siduron in fertilizers by flame ionization gas-liquid chromatography.
January 1974, Journal - Association of Official Analytical Chemists,
J G Gerber, and J S Barnes, and A S Nies
Incorporation and distribution of dihomo-gamma-linolenic acid, arachidonic acid, and eicosapentaenoic acid in cultured human keratinocytes.
February 1986, Prostaglandins,
J G Gerber, and J S Barnes, and A S Nies
The metabolism of dihomo-gamma-linolenic acid in man.
February 1979, Lipids,
Copied contents to your clipboard!