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Free grafts of the extensor digitorum longus (EDL) muscle of the rat are invaded by regenerating nerve fibers during the second postoperative week. Neuromuscular junctions are established during the third week. Levels of choline acetyltransferase (CAT) activity parallel the invasion of the graft by nerve fibers, but this activity never returns to control levels. We conclude that incomplete reinnervation of free muscle grafts may be a factor in the failure of such grafts to reattain the mass of control muscles.
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