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The hairy-cells (HC) of 10 patients with hairy-cell leukaemia were studied with several techniques to evaluate their phagocytic potential. Mononuclear cells from normal donors and from patients with acute monocytic leukaemia served as controls. Light microscopically HC seemed to have ingested bacteria or latex particles. Treatment of the cells with lysostaphin, an enzyme that kills extracellular Staphylococcus aureus, showed that almost all 'ingested' bacteria were extracellular. Lanthanum nitrate, added during the fixation procedure for electron microscopy, stained both the outer cell membrane and the membranes of the 'phagosomes' of the HC, also indicating that the 'ingested' particles were extracellular. HC showed no increased oxygen consumption on exposure to bacteria in the presence of serum. Furthermore, HC showed no lysozyme or peroxidase activity, whereas non-specific esterase activity was much weaker than in monocytes. These findings, which show that HC are essentially non-phagocytic, constitute strong evidence against a monocytic origin of the malignant cells of hairy-cell leukaemia.
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
January 1982,
Virchows Archiv. B, Cell pathology including molecular pathology,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
January 1981,
Sangre,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
May 1979,
Virchows Archiv. B, Cell pathology including molecular pathology,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
December 1977,
Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
January 1977,
Blood,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
January 1977,
Science (New York, N.Y.),
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
October 2007,
Immunological reviews,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
November 1981,
American journal of clinical pathology,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
February 1975,
The American journal of pathology,
J Jansen, and
C J Meijer, and
P van der Valk, and
W C de Bruyn, and
P C Leijh, and
G J den Ottolander, and
R van Furth
July 1980,
Journal of comparative pathology,
