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A method is described for producing banding patterns with methyl green-pyronin (MGP) stain in chromosomes of fibrosarcoma cells. 1) The stain was made by mixing equal volumes of 2% aqueous pyronin G, 2% aqueous methyl green, distilled water, and 0.1 M acetate buffer (pH 5.7). 2) Treatment with colcemide and hypotonic KCl (0.075 M) was performed as usual. 3) Metaphase chromosomes were prepared using the flame-drying technique and treated with 0.25% trypsin at 37 C for 45 to 90 seconds. Before staining, the slides were rinsed in PBS, in distilled water, and then were dipped in 0.05 M acetate buffer. 4) Chromosomes were stained for more than 20 minutes, rinsed in distilled water, and hot-air dried. Satisfactory results were obtained in uncontracted metaphase chromosomes. MGP stain has the advantage of permitting much longer trypsin treatment and staining time than the trypsin-Giemsa method while providing satisfactory banding patterns.
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
June 1983,
The Histochemical journal,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
August 1952,
Journal of the National Cancer Institute,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
September 1952,
Stain technology,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
January 1950,
The Journal of general physiology,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
August 1980,
The British journal of venereal diseases,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
January 1972,
Stain technology,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
January 1976,
Tissue & cell,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
March 1971,
Stain technology,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
September 1955,
Stain technology,
K Tabuchi, and
T Saiga, and
T Adachi, and
O Midorikawa
April 1977,
Medical laboratory sciences,
