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Antigen changes during spore formation in Bacillus cereus. 1964

A Baillie, and J R Norris

Baillie, Ann (University of Glasgow, Glasgow, Scotland), and John R. Norris. Antigen changes during spore formation in Bacillus cereus. J. Bacteriol. 87:1221-1226. 1964.-Ultrasonic extracts of Bacillus cereus cells harvested at different stages in spore formation were analyzed by immunoelectrophoresis with antisera prepared by injecting similar extracts into rabbits. Seven antigens resistant to heating at 80 C for 10 min were detected in extracts of young vegetative cells, and there were at least eight additional thermolabile antigens present at this stage. During spore formation, many of the thermolabile antigens disappeared from the cells and new antigens could be detected. Mature spores contained four of the heat-resistant antigens present in young vegetative cells and an additional five heat-resistant antigens. Only three thermolabile antigens were detected in spore extracts.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D000941 Antigens Substances that are recognized by the immune system and induce an immune reaction. Antigen
D001409 Bacillus cereus A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
D013170 Spores The reproductive elements of lower organisms, such as BACTERIA; FUNGI; and cryptogamic plants. Spore
D014465 Ultrasonics A subfield of acoustics dealing in the radio frequency range higher than acoustic SOUND waves (approximately above 20 kilohertz). Ultrasonic radiation is used therapeutically (DIATHERMY and ULTRASONIC THERAPY) to generate HEAT and to selectively destroy tissues. It is also used in diagnostics, for example, ULTRASONOGRAPHY; ECHOENCEPHALOGRAPHY; and ECHOCARDIOGRAPHY, to visually display echoes received from irradiated tissues. Ultrasonic

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