Biomaterial Database

The primary isolation of Leishmania donovani from Iraq on different culture media. 1979

M B Rassam, and S A Al-Mudhaffar

Leishmania donovani was isolated by culturing bone marrow aspirates from kala-azar patients, on three types of culture media. The isolation was successful in 88% of the cases. The effects of pH, D-glucose, L-proline and antibiotic concentration in the isolation were investigated. The semi-solid medium used (pH 7.4) with L-proline, was better than the modified NNN media for the primary isolation.

UI	MeSH Term	Description	Entries
D007223	Infant	A child between 1 and 23 months of age.	Infants
D007493	Iraq	A country bordering the Persian Gulf, between Iran and Kuwait. The capital is Baghdad.	Republic of Iraq
D007891	Leishmania	A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.	Leishmania (Leishmania),Leishmania (Viannia),Leishmania leishmania,Leishmania viannia,Leishmania leishmanias,Leishmania viannias,Leishmanias,Leishmanias (Leishmania),Leishmanias (Viannia),leishmanias, Leishmania,viannias, Leishmania
D007898	Leishmaniasis, Visceral	A chronic disease caused by LEISHMANIA DONOVANI and transmitted by the bite of several sandflies of the genera Phlebotomus and Lutzomyia. It is commonly characterized by fever, chills, vomiting, anemia, hepatosplenomegaly, leukopenia, hypergammaglobulinemia, emaciation, and an earth-gray color of the skin. The disease is classified into three main types according to geographic distribution: Indian, Mediterranean (or infantile), and African.	Black Fever,Kala-Azar,Fever, Black,Kala Azar,Visceral Leishmaniasis
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

M B Rassam, and S A Al-Mudhaffar

Evaluation of different culture media for isolation of Leishmania donovani.

December 1980, The Journal of communicable diseases,

M B Rassam, and S A Al-Mudhaffar

In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation.

January 2017, The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale,

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Isolation of lipophosphoglycans from Leishmania donovani amastigotes.

October 1996, Archives of biochemistry and biophysics,

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The metabolism of Leishmania donovani in culture.

February 1954, The Biochemical journal,

M B Rassam, and S A Al-Mudhaffar

Isolation and characterization of adenosine kinase from Leishmania donovani.

April 1987, The Journal of biological chemistry,

M B Rassam, and S A Al-Mudhaffar

Isolation and identification of coenzyme Q from Leishmania donovani.

May 1988, Molecular and biochemical parasitology,