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[Comparison of reactivities or uridine- and cytidine-2',3'-cyclophosphates as donors and uridine and cytidine as acceptors of phosphate under dinucleoside monophosphate synthesis catalyzed by RNAase A]. 1979

A P Kavunenko, and T N Kalacheva, and N S Tikhomirova-Sidorova

In order to determine the relative activity of pyrimidine nucleoside-2',3'-cyclophosphates as donors and nucleosides as acceptors of phosphate in the reaction of the internucleotide bond formation catalyzed by RNAase A (EC 3.4.1.22), a comparative synthesis of dinucleoside monophosphates UpU, UpC, CpU and CpC at three different enzyme concentrations (20, 40 and 70 mkg/ml) and two temperatures (0 degrees and -15 degrees) was carried out. The conversion rate of donor (U greater than p and C greater than p) during the synthesis and in the competitive reaction of hydrolysis strongly depends on the type of acceptor activity as compared to uridine. Based on the data of synthesis and simultaneous hydrolysis of U greater than p and C greater than p it may be concluded that in the both cases the latter donor is more reactive. The approaches to the determination of the substrate activity of the donors and acceptors for the evaluation of optimal conditions of the dinucleoside monophosphate synthesis depending on the donor--acceptor combination are discussed.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D009712 Nucleotides, Cyclic Cyclic Nucleotide,Cyclic Nucleotides,Nucleotide, Cyclic
D003562 Cytidine A pyrimidine nucleoside that is composed of the base CYTOSINE linked to the five-carbon sugar D-RIBOSE. Cytosine Ribonucleoside,Cytosine Riboside,Ribonucleoside, Cytosine,Riboside, Cytosine
D003597 Cytosine Nucleotides A group of pyrimidine NUCLEOTIDES which contain CYTOSINE. Cytidine Phosphates,Nucleotides, Cytosine,Phosphates, Cytidine
D004720 Endonucleases Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-. Endonuclease
D012260 Ribonucleases Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-. Nucleases, RNA,RNase,Acid Ribonuclease,Alkaline Ribonuclease,Ribonuclease,RNA Nucleases,Ribonuclease, Acid,Ribonuclease, Alkaline
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014500 Uracil Nucleotides A group of pyrimidine NUCLEOTIDES which contain URACIL. Uridine Phosphates,Uridine Nucleotides,Nucleotides, Uracil,Nucleotides, Uridine,Phosphates, Uridine
D014529 Uridine A ribonucleoside in which RIBOSE is linked to URACIL. Allo-Uridine,Allouridine,Allo Uridine

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