Biomaterial Database

A simple diagnostic milk medium for Pseudomonas aeruginosa. 1970

M R Brown, and J H Foster

An agar medium containing 10% defatted milk has been tested as a diagnostic medium for Pseudomonas aeruginosa and, in particular, for differentiating between P. aeruginosa and P. fluorescens. Only P. aeruginosa colonies gave clear zones due to hydrolysis of casein together with diffused green pigment. Results on milk agar correlated well with the pattern of results from a variety of conventional tests used to identify this organism. Pigment production of P. aeruginosa on milk agar was better than on special media commonly used to enhance this characteristic. Routine diagnosis of P. aeruginosa is recommended by streaking on a solid medium containing 10% defatted milk granules, 25% nutrient broth, and 2% agar, and examining for clear zones and pigment after 24 hours' incubation.

UI	MeSH Term	Description	Entries
D008892	Milk	The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals.	Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010619	Phenazines
D010860	Pigments, Biological	Any normal or abnormal coloring matter in PLANTS; ANIMALS or micro-organisms.	Biological Pigments
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D002374	Catalase	An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.	Catalase A,Catalase T,Manganese Catalase,Mn Catalase
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005780	Gelatin	A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.	Gelafusal
D005942	Gluconates	Derivatives of gluconic acid (the structural formula HOCH2(CHOH)4COOH), including its salts and esters.	Copper Gluconate,Gluconate, Copper
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia