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Control of in vitro cytotoxicity of positively charged liposomes. 1979

E A Panzner, and V K Jansons

The parameters which influence the in vitro cytotoxicity of positively charged liposomes for L 1210 cells were analyzed. The cytotoxicity was liposome/cell ratio-dependent. It also depended upon the mole fractions of stearylamine (SA) to phosphatidylcholine (PC). There was no difference between the cytotoxicity of unilamellar and multilamellar vesicles but the cytotoxic effect of free SA was about 4 times greater than that of liposome incorporated SA at a molar ratio of 1:4, SA:PC, respectively. The process which resulted in cell death was irreversible after 60 min of cell-liposome contact. The simultaneous presence of neutral liposomes or of positively charged liposomes with a lesser charge density decreased the cytotoxic effect of liposomes with a higher SA content. The cytotoxicity could be decreased by trypsinization of cells following exposure to liposomes while treatment of cells with trypsin prior to the exposure to positively charged liposomes had no effect on the subsequent cytotoxicity. The cytotoxicity was also decreased if cells were incubated in the presence of sodium azide. The usual concentration of serum (10%) present in the growth medium had no effect on the cytotoxicity while preincubation of cells with liposomes in 80% serum resulted in full protection. The protective effect of serum could be replaced by the albumin fraction.

UI MeSH Term Description Entries
D007939 Leukemia L1210 An experimental LYMPHOCYTIC LEUKEMIA of mice. Leukemia L 1210,L 1210, Leukemia,L1210, Leukemia
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D010713 Phosphatidylcholines Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a CHOLINE moiety. Choline Phosphoglycerides,Choline Glycerophospholipids,Phosphatidyl Choline,Phosphatidyl Cholines,Phosphatidylcholine,Choline, Phosphatidyl,Cholines, Phosphatidyl,Glycerophospholipids, Choline,Phosphoglycerides, Choline
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004563 Electrochemistry The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes. Electrochemistries
D000588 Amines A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed) Amine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001386 Azides Organic or inorganic compounds that contain the -N3 group. Azide

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