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1. Human alpha(2)-macroglobulin was prepared from a fraction obtained during the large-scale separation of normal human plasma proteins for clinical use. 2. Sedimentation-equilibrium measurements indicated a molecular weight of 725000. A value of 18.1S was obtained for s(0) (20,w). 3. The dissociation that occurs in the pH range 4.5-2.5 and in the region of neutrality in urea-containing solutions is consistent with a dimeric structure of the molecule. 4. The effects of the thiol reagents mercaptoethanol, mercaptoethylamine and N-acetylcysteine were investigated over a range of experimental conditions. Distinct components having sedimentation coefficients of 15, 12 and 8.5S were identified. 5. Conditions were found under which limited reduction with thiol liberated a subunit with a molecular weight approximately one-quarter of that of the intact molecule. This subunit retains the serological specificity of the whole molecule.
J M Jones, and
J M Creeth, and
R A Kekwick
June 1982,
Biochimica et biophysica acta,
J M Jones, and
J M Creeth, and
R A Kekwick
February 1977,
The Journal of biological chemistry,
J M Jones, and
J M Creeth, and
R A Kekwick
January 1972,
Biochimica et biophysica acta,
J M Jones, and
J M Creeth, and
R A Kekwick
February 1968,
Biochimica et biophysica acta,
J M Jones, and
J M Creeth, and
R A Kekwick
January 1977,
Biochimie,
J M Jones, and
J M Creeth, and
R A Kekwick
July 1984,
The Journal of biological chemistry,
J M Jones, and
J M Creeth, and
R A Kekwick
September 1973,
The Journal of experimental medicine,
J M Jones, and
J M Creeth, and
R A Kekwick
July 1982,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
J M Jones, and
J M Creeth, and
R A Kekwick
April 1993,
Archives of biochemistry and biophysics,
J M Jones, and
J M Creeth, and
R A Kekwick
April 1992,
Biochemical and biophysical research communications,
