Biomaterial Database

Electrogenic sodium pump in smooth muscle cells of the guinea-pig's taenia coli. 1971

R Casteels, and G Droogmans, and H Hendrickx

1. The changes of the membrane potential, of the K equilibrium potential, and of the membrane conductance during K accumulation by K-depleted tissues have been studied. Three subsequent characteristic periods can be described.2. Readmission of 5.9 mM-K after complete depletion results in a rapid extrusion of Na and uptake of K, and in a rapid hyperpolarization of the cells. Initially the time course of the K equilibrium potential and the membrane potential are similar except in propionate solution. This initial period is characterized by a high membrane conductance. No change of membrane potential occurs if 10(-5)M ouabain is present.3. After 5-7 min the membrane potential becomes more negative than the K equilibrium potential. The difference between both values is larger in solutions containing propionate or in hypertonic solutions. This second phase of the recovery period is characterized by a progressive decrease of the membrane conductance.4. In a third phase both the membrane potential and the membrane resistance return to their steady-state value.5. If the external K concentration in the recovery solution is increased, the maximal hyperpolarization is less and has a shorter duration. A decrease of the temperature of the recovery solution results in a slower initial rate of repolarization and in a decrease of the maximal value of the hyperpolarization.6. These observations demonstrate the existence of an electrogenic sodium pump in smooth muscle cells during stimulation of the Na pump. An analysis of the experimental data obtained under steady-state conditions in normal Krebs solution suggests that also under these conditions an electrogenic Na pump might take part in the maintenance of the resting potential.

UI	MeSH Term	Description	Entries
D006982	Hypertonic Solutions	Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Hypertonic Solution,Solution, Hypertonic,Solutions, Hypertonic
D008564	Membrane Potentials	The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).	Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D009566	Nitrates	Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.	Nitrate
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D011188	Potassium	An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D011422	Propionates	Derivatives of propionic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxyethane structure.	Propanoate,Propanoic Acid,Propionate,Propanoates,Propanoic Acid Derivatives,Propanoic Acids,Propionic Acid Derivatives,Propionic Acids,Acid, Propanoic,Acids, Propanoic,Acids, Propionic,Derivatives, Propanoic Acid,Derivatives, Propionic Acid
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D003106	Colon	The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.	Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D004553	Electric Conductivity	The ability of a substrate to allow the passage of ELECTRONS.	Electrical Conductivity,Conductivity, Electric,Conductivity, Electrical