Biomaterial Database

Medium for isolation of Yersinia enterocolitica. 1979

M V Dudley, and E B Shotts

A new differential plate medium, CAL (cellobiose-arginine-lysine) agar, was developed and evaluated for the isolation of Yersinia enterocolitica. For all but two isolates tested, distinctive colonies were formed on CAL agar within 40 h of incubation at 25 degrees C. These colonies were readily recognized and isolated from a mixed inoculum containing 10 other bacteria commonly found in water and fecal specimens. Y. enterocolitica was presumptively identified based on colonial morphology from 9 of 10 fecal cultures containing the organism when inoculated to CAL agar and incubated for 40 h at 25 degrees C. No false-positive identification were made. The medium has been shown to be as sensitive as MacConkey agar in supporting growth of Y. enterocolitica, and it can be used in broth from for enumeration of the organism from water and liquid specimens. The medium is easily made and does not need to be autoclaved.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001419	Bacteria	One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.	Eubacteria
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D014871	Water Microbiology	The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.	Microbiology, Water
D015007	Yersinia	A genus of gram-negative, facultatively anaerobic rod- to coccobacillus-shaped bacteria that occurs in a broad spectrum of habitats.