Biomaterial Database

Determination of alkaline phosphatase (EC 3.1.3.1) on the SMAC using diethanolamine in a modified manifold. 1979

S I Hansen, and E West-Nielsen, and J Lyngbye

The SMAC method for alkaline phosphatase was modified inorder to determine the enzyme activity according to the Scandinavian recommended method [1]. The method is not strictly 'Scandinavian', but the correlation with the strict Scandinavian method performed on the LKB 8600 Reaction rate analyzer was excellent. The modified method was linear up to at least 2000 U/l.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D004983	Ethanolamines	AMINO ALCOHOLS containing the ETHANOLAMINE; (-NH2CH2CHOH) group and its derivatives.	Aminoethanols
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D001322	Autoanalysis	Method of analyzing chemicals using automation.	Autoanalyses
D012537	Scandinavian and Nordic Countries	NORWAY; SWEDEN; DENMARK; ICELAND; and FINLAND.	Scandinavia,Nordic Countries