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The authors propose a modification of the method of use of the API 20E system permitting more rapid identification of Enterobacteriaceae within six hours (3 hours preculture and 3 hours incubation on an API 20E plate) it was possible to identify correctly 67% of 192 strains studied at species level and 75.5% studied at generic level. One may note four mistakes (2.1%) of which 3 were minor, (species within the same genus). The construction of a base of numerical data adapted to the technic within six hours would no doubt permit us to reduce the percentage undetermined.
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