-transparent.png){kind=logo}
A specific oxidoreductase converting aclacinomycin A to a new analog, aclacinomycin Y, was purified to apparent homogeneity from the culture filtrate of aclacinomycin-producing microorganisms. The isolated enzyme was a weakly acidic protein (isoelectric point, 5.9) with a molecular weight of about 72,000. The enzymatic reaction requires molecular oxygen and has a pH optimum at 5.5. The enzyme catalyzed an oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. Studies of substrate specificity revealed that the enzyme is an oxidoreductase capable of modifying anthracyclic triglycosides by oxidizing their terminal sugars.
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
November 1983,
The Journal of antibiotics,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
June 1974,
Archives of biochemistry and biophysics,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
July 2000,
Biochimica et biophysica acta,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
January 2003,
Communications in agricultural and applied biological sciences,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
June 1992,
The Journal of antibiotics,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
October 1998,
Applied and environmental microbiology,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
May 1996,
Bioscience, biotechnology, and biochemistry,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
February 2007,
Acta crystallographica. Section D, Biological crystallography,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
January 2022,
Biochemical and biophysical research communications,
A Yoshimoto, and
T Ogasawara, and
I Kitamura, and
T Oki, and
T Inui, and
T Takeuchi, and
H Umezawa
August 1997,
Biochemical Society transactions,
