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A nuclear framework structure has been obtained from isolated interphase nuclei of Physarum polycephalum by extraction with 2.5 M NaCl and subsequent digestion with DNase. Whole-mount electron micrographs showed a nuclear lamina containing residual pore structures associated with the fibrous internal matrix. The matrix was continuous with fibrillar remnants of the nucleolus. The structure was shown to consist of 2 major polypeptides of 23,000 and 36,500 Daltons as well as 30 to 40 minor polypeptides of various molecular weight classes. The 2 major polypeptides were also prominent in preparations of the residual nucleolar material, suggesting that matrix proteins are common to both structures. The predominance of low-molecular-weight polypeptides in Physarum nuclear matrix suggests that there may be significant differences in composition of nuclear structural proteins between lower and higher eukaryotes.
K R Mitchelson, and
A G Bekers, and
F Wanka
November 1969,
Archives of biochemistry and biophysics,
K R Mitchelson, and
A G Bekers, and
F Wanka
June 1960,
Experimental cell research,
K R Mitchelson, and
A G Bekers, and
F Wanka
December 1970,
Genetics,
K R Mitchelson, and
A G Bekers, and
F Wanka
October 1966,
Journal of bacteriology,
K R Mitchelson, and
A G Bekers, and
F Wanka
January 1972,
Tissue & cell,
K R Mitchelson, and
A G Bekers, and
F Wanka
May 1972,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
K R Mitchelson, and
A G Bekers, and
F Wanka
August 1978,
Biochimica et biophysica acta,
K R Mitchelson, and
A G Bekers, and
F Wanka
May 1978,
Archives internationales de physiologie et de biochimie,
K R Mitchelson, and
A G Bekers, and
F Wanka
February 1978,
Biochimica et biophysica acta,
K R Mitchelson, and
A G Bekers, and
F Wanka
September 1982,
Biokhimiia (Moscow, Russia),
