-transparent.png){kind=logo}
Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays. The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH(4))(2)SO(4), respectively. Both enzymes prefer maize nuclear DNA as a template; they are also more active in the presence of Mg(++) than Mn(++) and are inhibited by (NH(4))(2)-SO(4) or KCl. Neither enzyme is inhibited by rifamycin SV. Enzyme II is strongly inhibited by alpha-amanitin, whereas enzyme I is not significantly affected. Their ability to use native and denatured DNA as templates varies according to the extent and method of purification of the polymerase. Furthermore, enzyme II can be resolved by DEAE-chromatography or glycerol-gradient centrifugation into two components, one of which prefers native DNA, while the other prefers denatured DNA.
G C Strain, and
K P Mullinix, and
L Bogorad
January 1975,
Annual review of biochemistry,
G C Strain, and
K P Mullinix, and
L Bogorad
October 1979,
Proceedings of the National Academy of Sciences of the United States of America,
G C Strain, and
K P Mullinix, and
L Bogorad
January 1993,
Methods in molecular biology (Clifton, N.J.),
G C Strain, and
K P Mullinix, and
L Bogorad
June 2009,
Genes & development,
G C Strain, and
K P Mullinix, and
L Bogorad
March 1990,
Genes & development,
G C Strain, and
K P Mullinix, and
L Bogorad
May 1999,
The Plant cell,
G C Strain, and
K P Mullinix, and
L Bogorad
January 1976,
The ... Symposium. Society for Developmental Biology. Symposium,
G C Strain, and
K P Mullinix, and
L Bogorad
January 1976,
The Journal of clinical investigation,
G C Strain, and
K P Mullinix, and
L Bogorad
April 2010,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
G C Strain, and
K P Mullinix, and
L Bogorad
October 1971,
Proceedings of the National Academy of Sciences of the United States of America,
