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Media and methods for phage-typing mycobacteria. 1966

W B Redmond, and D M Ward

Media widely used for primary isolation, for drug-sensitivity tests and for type differentiation of mycobacteria have been tested for use in phage studies on the tubercle bacilli. Modifications of each of these media provided some improvement in phage activity on some strains of mycobacteria but not on others. During these studies it was observed that certain substances augmented phage activity, while other substances were found to have the opposite effect. On the basis of these results, a new medium has been formulated. This medium can be used without agar as a liquid medium for growth of the mycobacteria or with agar as a solid medium for isolated plaques and routine test dilution (RTD) spots. An outline of a pilot phage-typing test on three species of Mycobacterium is included.

UI MeSH Term Description Entries
D009160 Mycobacteriophages Viruses whose host is one or more Mycobacterium species. They include both temperate and virulent types. Mycobacteriophage
D009161 Mycobacterium A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts. Mycobacteria
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D001434 Bacteriophage Typing A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages. Phage Typing,Typing, Bacteriophage,Typing, Phage

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