-transparent.png){kind=logo}
Soluble antigens from Histoplasma capsulatum in the mycelial and yeast phase were purified by gel filtration, fixed onto paper discs, and employed in an indirect immunofluorescence procedure to detect antibody in sera from individuals infected with H. capsulatum. The elution patterns of crude histoplasmin passed through Sephadex G-200 revealed two minor peaks of protein showing immunofluorescence, complement fixing, and precipitating-antigen activity. A large peak containing the pigment and other low molecular weight materials showed no serological activity. A polysaccharide antigen obtained from fragmented, deproteinized yeast-phase cells was reactive in the fluorescent-antibody test but showed no antigen activity in complement fixation or precipitin tests. Although certain sera from culturally proven cases of blastomycosis, coccidioidomycosis, and cryptococcosis reacted with the purified Histoplasma antigens, preliminary evaluation indicated that the immunofluorescence technique may be of value as a screening procedure for the serodiagnosis of histoplasmosis.
W A Hook, and
E H Fife
March 1964,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
W A Hook, and
E H Fife
July 1976,
The American review of respiratory disease,
W A Hook, and
E H Fife
March 1979,
Mycopathologia,
W A Hook, and
E H Fife
March 1989,
Infection and immunity,
W A Hook, and
E H Fife
December 1965,
The American review of respiratory disease,
W A Hook, and
E H Fife
January 1983,
Methods in enzymology,
W A Hook, and
E H Fife
January 1992,
Revista cubana de medicina tropical,
W A Hook, and
E H Fife
September 1948,
Journal of immunology (Baltimore, Md. : 1950),
W A Hook, and
E H Fife
October 2007,
Clinical and vaccine immunology : CVI,
