BIOMAT Database Logo BIOMAT Database Logo

Streptolysin O: sedimentation coefficient and molecular weight determinations. 1969

D E Van Epps, and B R Andersen

The sedimentation coefficient of streptolysin O as determined by sucrose density gradient ultracentrifugation is 3.7S. An approximate molecular weight of 60,500 was calculated from the sedimentation velocity, and a similar value was obtained by Sephadex gel filtration. There was no measurable difference in the sedimentation coefficient of streptolysin O in either the active or reversibly inactive forms, indicating that there were at most only minor conformational differences between the two forms.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D002499 Centrifugation, Density Gradient Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D013301 Streptolysins Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS. Streptolysin
D014461 Ultracentrifugation Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Related Publications

D E Van Epps, and B R Andersen
Simultaneous rapid estimation of sedimentation coefficient and molecular weight.
April 1980, Biophysical chemistry,
D E Van Epps, and B R Andersen
Molecular-weight determinations during the approach to sedimentation equilibrium.
December 1956, Archives of biochemistry and biophysics,
D E Van Epps, and B R Andersen
Studies on human IgD. I. Molecular weight and sedimentation coefficient.
May 1969, Immunochemistry,
D E Van Epps, and B R Andersen
Relationship between the sedimentation coefficient and molecular weight of bacteriophages.
June 1969, The Journal of general virology,
D E Van Epps, and B R Andersen
Correlation between sedimentation coefficient and molecular weight of denatured DNA.
December 1974, Biochimica et biophysica acta,
D E Van Epps, and B R Andersen
PURIFICATION AND CHARACTERIZATION OF MILK LIPASE. III. SEDIMENTATION COEFFICIENT, DIFFUSION COEFFICIENT AND MOLECULAR WEIGHT.
September 1963, Enzymologia,
D E Van Epps, and B R Andersen
Ultracentrifugal studies in capillary cells. II. Sedimentation equilibrium molecular weight determinations.
June 1972, Analytical biochemistry,
D E Van Epps, and B R Andersen
Molecular weight determinations of o-raffinose-polymerized human hemoglobin.
January 1992, Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology,
D E Van Epps, and B R Andersen
Sedimentation coefficient and minimum molecular weight of extracellular hemoglobin of Glossoscolex paulistus (Oligochaeta).
January 1988, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
D E Van Epps, and B R Andersen
Molecular Weight Determinations.
August 1961, Science (New York, N.Y.),
Copied contents to your clipboard!