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Light microscopic studies have been carried out on the attachment and morphological responses of washed human platelets in serum-free medium to fibrinogen-coated, collagen-coated and uncoated tissue culture plastic substrata. Platelets were observed to attach to the substratum, extend filipodia and undergo spreading. Subsequently, lysis of platelets occurred. On uncoated tissue culture plastic substrata, the addition of cold insoluble globulin to the incubations had no effect on the above morphological changes. On the other hand, on the protein coated substrata, there was very little platelet spreading or lysis without the addition of cold insoluble globulin.
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