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An extract was prepared from rabbit reticulocyte ribosomes after treatment with potassium chloride as described by Miller, Hamada, Yang, Cohen & Schweet (1967). This extract has been shown to convert monoribosomes into polyribosomes during protein synthesis in vitro (Cohen, 1968). The nature of this extract was studied in greater detail. Centrifugation of the extract through a sucrose density gradient separated the activity into a fast-sedimenting fraction. The two fractions were shown to have different functions in stimulating cell-free protein synthesis and their active components were shown to be protein or partly protein in nature. Each fraction was analysed by electrophoresis and in the analytical ultracentrifuge. It was concluded that the active component in the fast-sedimenting fraction had a sedimentation coefficient of 15.5s and that of the slow-sedimenting fraction 10.5s.
B B Cohen
April 1971,
European journal of biochemistry,
B B Cohen
August 1990,
Biochimica et biophysica acta,
B B Cohen
May 1968,
Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimentelle Chirurgie,
B B Cohen
November 1965,
The Biochemical journal,
B B Cohen
January 1980,
Acta virologica,
B B Cohen
November 1972,
The Biochemical journal,
B B Cohen
December 1969,
Nature,
B B Cohen
May 1974,
Biochemical and biophysical research communications,
B B Cohen
January 1979,
Methods in enzymology,
B B Cohen
January 1979,
Methods in enzymology,
