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A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.
M S Parshley, and
J M Cerreta, and
I Mandl, and
J A Fierer, and
G M Turino
January 1975,
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M S Parshley, and
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November 1988,
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M S Parshley, and
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January 2010,
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M S Parshley, and
J M Cerreta, and
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September 1984,
Experimental cell research,
M S Parshley, and
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J A Fierer, and
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M S Parshley, and
J M Cerreta, and
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J A Fierer, and
G M Turino
January 1985,
Experimental cell biology,
M S Parshley, and
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I Mandl, and
J A Fierer, and
G M Turino
March 1992,
Experimental cell research,
