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We developed a new procedure for the rapid and gentle isolation of the human complement component C6 comparable to that described previously for C9. The procedure is based on affinity chromatography. As a first step, C6 is immunoabsorbed on insolubilized anti-C6 antibodies. These antibodies were derived from C6-defective rabbits (Freiburg strain). C6 was eluted with 3 M thiocyanate, pH 7.2, with a recovery of 15--23% of its hemolytic activity and a more than 270--fold purification. Impurities were removed in a second step by an "anti-impurity" column. The final product yielded a 12% recovery of the hemolytic activity and the purification factor was higher than 1300. The final product was homogeneous in SDS polyacrylamide and immunoelectrophoresis.
E W Rauterberg, and
G Hänsch, and
U Rother
November 1971,
Journal of immunology (Baltimore, Md. : 1950),
E W Rauterberg, and
G Hänsch, and
U Rother
September 1973,
Journal of immunology (Baltimore, Md. : 1950),
E W Rauterberg, and
G Hänsch, and
U Rother
February 1977,
Immunochemistry,
E W Rauterberg, and
G Hänsch, and
U Rother
January 1976,
Arthritis and rheumatism,
E W Rauterberg, and
G Hänsch, and
U Rother
January 1991,
Nucleic acids research,
E W Rauterberg, and
G Hänsch, and
U Rother
December 1989,
Biotechnology and applied biochemistry,
E W Rauterberg, and
G Hänsch, and
U Rother
September 1989,
The Journal of biological chemistry,
E W Rauterberg, and
G Hänsch, and
U Rother
July 1974,
Journal of immunology (Baltimore, Md. : 1950),
E W Rauterberg, and
G Hänsch, and
U Rother
March 1985,
Life sciences,
E W Rauterberg, and
G Hänsch, and
U Rother
April 1994,
Protein expression and purification,
