Biomaterial Database

Factors affecting the glycosphingolipid composition of murine tissues. 1970

L Coles, and J B Hay, and G M Gray

The effects of genetic strain, sex, age, and pathological state on the distribution and concentration of glycosphingolipids in mouse kidneys and livers were studied. The concentrations of glycosphingolipids and phospholipids in the kidneys and livers of different strains were compared. The major glycosphingolipid in the kidneys of male and female BALB/c, C3H/He, C57/BL, A, and C57xA (F(1) hybrid) mice was a sulfatide, monoglycosyl-(3-sulfate) ceramide; monoglycosyl ceramide was the major component in livers. The kidneys of males of all strains contained significant amounts of diglycosyl ceramide, but those of females contained, at most, only traces. Glycosphingolipid concentration in the male kidneys of C57/BL and C57xA (F(1) hybrid) was much higher than in the female and was also much higher than in the male kidneys of C3H/He, BALB/c, and A strains. The kidneys of "old" (36 wk) male and female C3H/He mice contained much higher proportions of monoglycosyl ceramide than 10-12-wk-old adults. The distributions of glycosphingolipids in kidneys of female C3H/He mice with BP8 ascites tumors and male C57xA (F(1) hybrid) mice with EL4 ascites tumors differed from those in the normal mice. An unknown lipid, present in all glycosphingolipid extracts from kidney and liver, was tentatively identified as cholesterol sulfate.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007942	Leukemia, Experimental	Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.	Experimental Leukemia,Experimental Leukemias,Leukemia Model, Animal,Leukemias, Experimental,Animal Leukemia Model,Animal Leukemia Models,Leukemia Models, Animal
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D009374	Neoplasms, Experimental	Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.	Experimental Neoplasms,Experimental Neoplasm,Neoplasm, Experimental
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D002554	Cerebrosides	Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D002855	Chromatography, Thin Layer	Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D005260	Female		Females