-transparent.png){kind=logo}
From 2000 sera with elevated total creatine kinase (EC 2.7.3.2) activity the sera of 6 patient showed a persistent high and anamnestically inexplicable creatine kinase activity level. The serum isoenzyme pattern was analyzed by four different methods. Electrophoresis revealed an atypical creatine activity band located between creatine kinase-MM and creatine kinase-MB. An adequate estimation of the persistent enzyme activity could only be achieved by immunotitration (immunoprecipitation), which identified the creatine kinase activity as due primarily to the isoenzyme creatine kinase-BB. Other methods (immunoinhibition and column chromatography) may lead to misinterpretations. The atypical serum creatine kinase-BB showed a higher molecular weight (Mr = 250,000) and altered substrate affinity as compared to native creatine kinase-BB. Both properties are attributable to the binding of creatine kinase-BB to immunoglobulins.
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
September 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
June 1982,
Clinical chemistry,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
March 1979,
Clinical chemistry,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
August 1979,
Clinical chemistry,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
November 1994,
Clinical and experimental dermatology,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
March 1983,
Clinical chemistry,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
September 1981,
Scandinavian journal of clinical and laboratory investigation,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
August 2005,
Military medicine,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
July 1983,
Clinical chemistry,
G Chemnitz, and
E Jockers-Wretou, and
E Schmidt, and
F W Schmidt, and
J Lobers
January 1984,
Clinical chemistry,
