BIOMAT Database Logo BIOMAT Database Logo

[Steroid-transforming enzymes from microorganisms. XII. Induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in Nocardia opaca]. 1979

C Hörhold, and E Hüller, and G Rose

17 alpha-Methyltestosterone and the corresponding 1(2)-dehydrocompound (Dianabol) are efficient inducers of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca. After a lag period of 4 hours the enzyme activity increases rapidly. During the induction the steroids are completely metabolized causing a drastical drop of specific enzyme activity. Using a fixed induction time the optimal steroid concentration and the temperature characteristic were found out. The influence of the concentration of the steroid water suspension on the induction effect is discussed to be dependent on the velocity of the dissolving of the steroid particles. Chloramphenicol and streptomycin are powerful inhibitors of the induction process.

UI MeSH Term Description Entries
D008696 Methandrostenolone A synthetic steroid with anabolic properties that are more pronounced than its androgenic effects. It has little progestational activity. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1188) Dehydromethyltestosterone,Metandienone,Methandienone,Dianabol,Metanabol,Nerobol
D008777 Methyltestosterone A synthetic hormone used for androgen replacement therapy and as an hormonal antineoplastic agent (ANTINEOPLASTIC AGENTS, HORMONAL). 17 beta Methyltestosterone,17 beta-Hydroxy-17-methyl-4-androsten-3-one,17 beta-Methyltestosterone,17-Epimethyltestosterone,17-alpha-Methyltestosterone,17alpha-Methyl-Testosterone,17alpha-Methyltestosterone,17beta-Hydroxy-17-methyl-4-androsten-3-one,17beta-Methyltestosterone,Android (Methyltestoterone),Android-10,Android-25,Android-5,Mesteron,Mesterone,Metandren,Methitest,Oreton,Testoviron,Testred,Virilon,17 Epimethyltestosterone,17 alpha Methyltestosterone,17 beta Hydroxy 17 methyl 4 androsten 3 one,17alpha Methyl Testosterone,17alpha Methyltestosterone,17beta Hydroxy 17 methyl 4 androsten 3 one,17beta Methyltestosterone,Android 10,Android 25,Android 5
D009615 Nocardia A genus of gram-positive, aerobic bacteria whose species are widely distributed and are abundant in soil. Some strains are pathogenic opportunists for humans and animals. Micropolyspora
D010088 Oxidoreductases The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9) Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D002701 Chloramphenicol An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106) Cloranfenicol,Kloramfenikol,Levomycetin,Amphenicol,Amphenicols,Chlornitromycin,Chlorocid,Chloromycetin,Detreomycin,Ophthochlor,Syntomycin
D004790 Enzyme Induction An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis. Induction, Enzyme
D013307 Streptomycin An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis. Estreptomicina CEPA,Estreptomicina Clariana,Estreptomicina Normon,Strepto-Fatol,Strepto-Hefa,Streptomycin Grünenthal,Streptomycin Sulfate,Streptomycin Sulfate (2:3) Salt,Streptomycin Sulphate,Streptomycine Panpharma,Strepto Fatol,Strepto Hefa
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

Related Publications

C Hörhold, and E Hüller, and G Rose
[Steroid transforming enzymes from microorganisms. V. Purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca and characterization of the prosthetic group].
January 1978, Zeitschrift fur allgemeine Mikrobiologie,
C Hörhold, and E Hüller, and G Rose
[Steroid-transforming enzymes from microorganisms. IX. Affinity chromatographic preparation and studies of the apoenzyme from a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca].
January 1979, Zeitschrift fur allgemeine Mikrobiologie,
C Hörhold, and E Hüller, and G Rose
[Steroid conjugating enzyme from microorganisms. VI. Purification of 4-en-o-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca by affinity chromatography].
September 1977, Journal of steroid biochemistry,
C Hörhold, and E Hüller, and G Rose
[Steroid-transforming enzymes from microorganisms. II. Purification of a 3-oxosteroid: (2,6-dichlorophenolindophenol) delta 1-oxidoreductase from Nocardia opaca by means of affinity chromatography].
January 1976, Zeitschrift fur allgemeine Mikrobiologie,
C Hörhold, and E Hüller, and G Rose
[Steroid metabolizing enzymes from microorganisms. VII. The nature of the biospecific binding to an affinity matrix-affinity chromatography of 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca].
June 1978, Journal of steroid biochemistry,
C Hörhold, and E Hüller, and G Rose
[Steroid converting enzymes from microorganisms. VIII. Effect of the organic solvent specific hydrophobicity of the binding on the affinity-matrix- affinity chromatography of a 4-en-3-oxo-steroid: (Acceptor)-1-en-oxidoreductase from Nocardia opaca].
July 1978, Journal of steroid biochemistry,
C Hörhold, and E Hüller, and G Rose
[Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca].
January 1979, Zeitschrift fur allgemeine Mikrobiologie,
C Hörhold, and E Hüller, and G Rose
Steroid transforming enzymes from microorganisms--III. Properties of 4-ene-3-oxosteroid-5alpha-reductase from Mycobacterium smegmatis.
June 1977, Journal of steroid biochemistry,
C Hörhold, and E Hüller, and G Rose
Steroid transforming enzymes from microorganisms: the reverse reaction of the steroid-1-dehydrogenase from Nocardia.
December 1980, Journal of steroid biochemistry,
C Hörhold, and E Hüller, and G Rose
Steroid transforming enzymes from microorganisms. IV. Purification and cofactor requirement of the 4-ene-3-oxosteroid-5alpha-reductase from Mycobacterium smegmatis.
April 1977, Journal of steroid biochemistry,
Copied contents to your clipboard!