Seven different tissues of the fish, Rasbora daniconius are analysed chromatographically for their free amino acid contents. Alanine and Cystine followed by Glycine and Glutamic acid are the most common amino acids found in the tissues. On the basis of sex, no apparent difference in amino acid pattern in various tissues is found. Up to 70% of the amino acids of the pools fall in the range of pH from 4.5-6.5. Thus it appears that the pools have some bearing on the maintenance of the tissue pH.