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The gel-filtration behaviour of calf thymus histone fraction F2(b) was studied at three different salt concentrations (0.01m-, 0.10m- and 1.00m-sodium chloride) and two different pH ranges (pH3-4 and pH6.7-7.1). Other histone fractions [F1, F2(a) and F3] were also utilized to assist interpretation of the data. It was found that the Stokes radius of histone fraction F2(b) was not significantly changed when the salt concentration was increased, implying that the aggregation of the individual histone molecules (Edwards & Shooter, 1969) resulted in only relatively minor changes in the hydrodynamic volume. Aggregation would appear to be due to the salting out of hydrophobic regions giving rise, in the aggregate, to a compact core of hydrophobic groups from which protrude the remaining basic parts of the molecule. Repulsion between charged groups on the basic regions of individual histone molecules would give the aggregate approximately spherical symmetry, the diameter of the aggregate approximating to the length of a single histone molecule.
P A Edwards, and
K V Shooter
April 1960,
Canadian journal of biochemistry and physiology,
P A Edwards, and
K V Shooter
January 1965,
Bulletin de la Societe de chimie biologique,
P A Edwards, and
K V Shooter
August 1973,
FEBS letters,
P A Edwards, and
K V Shooter
May 1968,
European journal of biochemistry,
P A Edwards, and
K V Shooter
January 1977,
Folia biologica,
P A Edwards, and
K V Shooter
November 1970,
Biochimica et biophysica acta,
P A Edwards, and
K V Shooter
November 1967,
The Biochemical journal,
P A Edwards, and
K V Shooter
January 1972,
Biochimie,
P A Edwards, and
K V Shooter
August 1955,
The Journal of biological chemistry,
P A Edwards, and
K V Shooter
April 1971,
FEBS letters,
