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Anti-d,l-methadone antibodies were produced in rabbits immunized with d,l-methadol-hemisuccinate thyroglobulin conjugate. Using the antiserum, a radioimmunoasay (RIA) for determination of d,l-methadone in human serum has been developed and is described. Concentration of d,l-methadone of 1.4 pmol in a native serum sample (volume 0.1 ml or less) could be measured directly by RIA. The antibodies crossreact 100% with d,l-methadone, 50% with d-methadone, 50% with l-methadone and 100% with alpha-d-methadol. No crossreactivity was found with alpha 1-methadol, morphine, meperidine, dextropropoxyphene, 2-ethyl-5-methyl-3,3-diphenyl-l-pyrroline and 2-ethylidene-l, 5-dimethyl-3,3-diphenylpyrrolidene. High sensitivity and small sample requirements make this method suitable for future monitoring of patients on methadone maintenance and for studies where other procedures have lack of sensitivity.
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
October 1980,
The Journal of pharmacology and experimental therapeutics,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
August 1981,
Klinische Wochenschrift,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
November 1977,
Science (New York, N.Y.),
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
January 1992,
Chirality,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
January 1975,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
July 1983,
Journal of pharmaceutical sciences,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
February 1970,
Gastroenterology,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
September 1981,
British journal of cancer,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
January 1989,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
F Bartos, and
G D Olsen, and
R N Leger, and
D Bartos
April 1987,
Acta neurologica Scandinavica,
