A simple method for beveling micropipettes for intracellular recording and current injection. 1977

M Tauchi, and R Kikuchi

A simple equipment for beveling intracellular microelectrodes has been made by modifying a conventional turntable. An agar disc containing ceric oxide polishing compound which was put on a flat glass disc was found to be suitable for beveling of Pyrex single- and double-barreled micropipette electrodes with a tip diameter larger than 0.1 mum. Although the beveled microelectrodes have not always been shown to give better cell penetration into the frog retinal rods than unbeveled find ones, better records could usually be obtained when electric currents were injected into cells, probably being due to their lower resistance, noise, tip potentials and coupling resistance between two barrels.

UI MeSH Term Description Entries
D007478 Iontophoresis Therapeutic introduction of ions of soluble salts into tissues by means of electric current. In medical literature it is commonly used to indicate the process of increasing the penetration of drugs into surface tissues by the application of electric current. It has nothing to do with ION EXCHANGE; AIR IONIZATION nor PHONOPHORESIS, none of which requires current. Iontophoreses
D008839 Microelectrodes Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed) Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005898 Glass Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.

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