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The content of globin coding sequences in nuclear RNA from chicken embryo red blood cells and chicken embryo brain was determined by hybridization with globin cDNA to be 0.27% for embryonic red blood cells and 0.001% for embryo brain, i.e. 0.37% of globin coding sequences relative to embryonic red blood cells. By transfusion of [3H]uridine labelled red blood cells it could be shown that the brain nuclear RNA preparation was contaminated by 0.41% RNA originating from blood cells. As this value is in the same range as the hybridization rate there is no evidence for globin transcripts in brain nuclear RNA. It has also not been possible to detect a short-lived globin mRNA precursor in pulse labelled brain nuclear RNA. In further experiments the RNAs were translated in an Ehrlich ascites cell-free system. No globin synthesis could be observed with brain RNA.
W Knöchel, and
E Kohnert-Stavenhagen
August 1978,
Cell,
W Knöchel, and
E Kohnert-Stavenhagen
January 1980,
Molekuliarnaia biologiia,
W Knöchel, and
E Kohnert-Stavenhagen
May 1977,
Biochimica et biophysica acta,
W Knöchel, and
E Kohnert-Stavenhagen
January 1975,
Acta embryologiae experimentalis,
W Knöchel, and
E Kohnert-Stavenhagen
October 1983,
Cell,
W Knöchel, and
E Kohnert-Stavenhagen
December 1985,
Proceedings of the National Academy of Sciences of the United States of America,
W Knöchel, and
E Kohnert-Stavenhagen
September 1976,
British medical bulletin,
W Knöchel, and
E Kohnert-Stavenhagen
August 1976,
Cell,
W Knöchel, and
E Kohnert-Stavenhagen
April 1973,
Proceedings of the National Academy of Sciences of the United States of America,
W Knöchel, and
E Kohnert-Stavenhagen
November 1974,
Annals of the New York Academy of Sciences,
