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Interferon production was induced in mouse Ehrlich ascites tumor cells by infection with Newcastle disease virus. The interferon produced was purified by precipitation with ammonium sulfate, chromatography on carboxymethyl-Sephadex, treatment with blue dextran and polyethylene glycol, gel filtration on Bio-Gel P-60 and Bio-Gel P-200, chromatography on phosphocellulose, isoelectric focusing, and chromatography on octyl-Sepharose. The specific activity of the product was 1.6 x 10(9) NIH mouse interferon reference standard units/mg of protein. Electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate indicated that the apparent molecular weight of the interferon-active material ranged from 25,000 to 35,000. As revealed by staining the gels with Coomassie brilliant blue, the interferon activity co-migrated with the major, broad protein band. Minor, stainable bands of proteins were free of interferon activity and their apparent molecular weight was smaller than 12,000.
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
May 1984,
Journal of biochemistry,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
January 1976,
Methods in enzymology,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
March 1966,
The Journal of biological chemistry,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
May 1972,
Biochimica et biophysica acta,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
November 1981,
Biochimica et biophysica acta,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
May 1979,
The Journal of biological chemistry,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
May 1982,
The Journal of biological chemistry,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
May 1967,
Proceedings of the National Academy of Sciences of the United States of America,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
January 1977,
Neoplasma,
M Kawakita, and
B Cabrer, and
H Taira, and
M Rebello, and
E Slattery, and
H Weideli, and
P Lengyel
January 1967,
Biochimica et biophysica acta,
