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Experiments are described leading to partial compensation of a deficiency in the enzyme hypoxanthine-guanine phosphoribosyltransferase in mutant cells by supplying the cells with exogenous purified enzymes. DEAE-dextran is an effective helper agent, whereas poly (L-lysine), lysolecithin and amphotericin B seem to inhibit the entry of the enzymes of their activity. Enzyme preparation from Chinese hamster was found to have different effects in different mutant cell lines. In mutant Chinese hamster cells, the electrophoretic activity pattern remains unchanged for the Chinese hamster enzyme, but changes progressively to faster-moving activity peaks for the human enzyme after several hours. The metabolic effect of the incorporated enzyme is in the range between 3 and 4% of the normal cellular enzyme activity which corresponds to a 10--20 fold increase of hypoxanthine-guanine phosphoribosyltransferase activity in the mutant cells.
M Strauss, and
M Theile, and
E Geissler
January 1975,
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M Strauss, and
M Theile, and
E Geissler
April 1971,
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M Strauss, and
M Theile, and
E Geissler
June 1975,
Cell,
M Strauss, and
M Theile, and
E Geissler
December 1976,
Science (New York, N.Y.),
M Strauss, and
M Theile, and
E Geissler
January 1975,
Cardiovascular Research Center bulletin,
M Strauss, and
M Theile, and
E Geissler
October 1973,
Biochemical medicine,
M Strauss, and
M Theile, and
E Geissler
September 1973,
Biochimica et biophysica acta,
M Strauss, and
M Theile, and
E Geissler
August 1994,
Journal of virology,
M Strauss, and
M Theile, and
E Geissler
January 1979,
Cytobios,
M Strauss, and
M Theile, and
E Geissler
February 1961,
Science (New York, N.Y.),
