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A reactive ATP analog, N6-(6-bromoacetamidohexyl)-AMP.PCP, was synthesized in an attempt to covalently label the binding sites for adenine nucleotides, especially ATP, of various enzymes which utilize adenine nucleotides as substrates, cofactors, inhibitors or allosteric effectors. This reagent rapidly inactivated rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GPD), myokinase (MK), and creatine kinase (CK) under very mild conditions. Adenine nucleotide substrates prevented the inactivation. In the case of GPD, complete inactivation was observed when 1 mol of the reagent per mol of enzyme subunit was incorporated into the enzyme. These results indicate that the present ATP analog may be useful as an affinity labeling reagent for various adenine nucleotide-dependent enzymes.
K Nagata, and
K Suzuki, and
K Imahori
April 1974,
European journal of biochemistry,
K Nagata, and
K Suzuki, and
K Imahori
June 2017,
Bioorganic & medicinal chemistry,
K Nagata, and
K Suzuki, and
K Imahori
October 1980,
The Journal of biological chemistry,
K Nagata, and
K Suzuki, and
K Imahori
May 1977,
Journal of biochemistry,
K Nagata, and
K Suzuki, and
K Imahori
August 2003,
Lipids,
K Nagata, and
K Suzuki, and
K Imahori
September 1975,
Biochemical and biophysical research communications,
K Nagata, and
K Suzuki, and
K Imahori
April 1977,
Journal of biochemistry,
K Nagata, and
K Suzuki, and
K Imahori
January 2008,
Bioconjugate chemistry,
K Nagata, and
K Suzuki, and
K Imahori
April 1978,
Biochemical and biophysical research communications,
K Nagata, and
K Suzuki, and
K Imahori
April 2021,
Environmental science and pollution research international,
