Biomaterial Database

Inhibition of staphylococcal alpha-toxin. The effect of aromatic polysulphonic acids on the lethal effect of alpha-toxin in mice. 1968

J P Arbuthnott, and I R Lominski, and M R Wright

1. Certain aromatic polysulphonic acids, previously tested for inhibition of the haemolytic activity of staphylococcal alpha-toxin, together with some additional related compounds, were tested as possible inhibitors of alpha-toxin in mice. 2. Compounds that inhibited the haemolytic activity of alpha-toxin at concentrations of 0.16mm or less [compounds (I), (II), (IV), (V), (VII) and (VIII)] were found to inhibit the lethal effect of alpha-toxin. 3. With the exception of compound (VIII), amounts of 1mg. were required to inhibit 4 LD(50) of toxin when the test compounds were premixed with alpha-toxin before injection; comparable inhibition with 0.3mg. of compound (VIII) was achieved without prolonged premixing. 4. Mixtures of alpha-toxin and compounds (I) and (II) containing an excess of test compound showed markedly diminished inhibitory activities. 5. The ;half-molecule' analogues of group 1 [compounds (III) and (XVIII)] were non-inhibitory. 6. Compounds (I)-(V), when administered separately from alpha-toxin by the same route (intraperitoneal), were active only when injected almost simultaneously with toxin, whereas compounds (VII) and (VIII) were strikingly inhibitory when injected 15min. before or after the toxin. 7. Compound (VIII) failed to inhibit the lethal effect of alpha-toxin when injected by a different route (intravenous).

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009281	Naphthalenes	Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.
D005260	Female		Females
D006461	Hemolysis	The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.	Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000992	Antitoxins	Antisera from immunized animals that is purified and used as a passive immunizing agent against specific BACTERIAL TOXINS.	Antitoxin
D001555	Benzene Derivatives	Organic compounds derived from BENZENE.	Derivatives, Benzene
D013210	Staphylococcus	A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
D013451	Sulfonic Acids	Inorganic or organic oxy acids of sulfur which contain the RSO2(OH) radical.	Sulfonic Acid,Acid, Sulfonic,Acids, Sulfonic
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor