1. The catecholamines adrenaline (A), noradrenaline (NA) and dopamine (DA) were determined in plasma samples of man and various animal species using a highly sensitive radioenzymatic method.2. Basal values were determined under conditions producing virtually no physical or psychic stress in blood obtained through acutely inserted venous catheters in human volunteers, rabbits and cows, through chronic indwelling catheters in cats and rats, and by cubital venipuncture in trained dogs.3. Basal values (pg/ml.) for A, NA, and DA were respectively 64, 203 and 98 in man, 73, 609 and 276 in cats, 166, 392 and 216 in rabbits, 56, 152 and 91 in cows, 204, 376 and 173 in dogs, and 175, 509, and 84 in SPF rats. The NA concentrations were always higher than those of A and DA.4. Gentle handling of rats for 30 sec greatly increased the levels of all catecholamines, especially of A. Even more marked rises were observed during and up to 5 min after restraint stress.5. Blood from the trunk of decapitated rats contained about 20 times more A and 3-4 times more DA and NA than venous blood from catheters in the absence of handling.6. Basal values of plasma catecholamines in small animals can only be obtained through indwelling catheters and in the absence of handling. Most of the previously reported values are too high and are experimental artifacts.