-transparent.png){kind=logo}
Double-stranded chicken lysozyme cDNA was synthesized from an oviduct mRNA fraction enriched for lysozyme mRNA. The ds-cDNA was inserted into the BamHI site of plasmid pBR322 using chemically synthesized DNA linker molecules containing the BamHI restriction endonuclease cleavage site. After bacterial transformation, colonies carrying lysozyme DNA were identified by hybridization with highly purified lysozyme cDNA. The 555 base pairs long cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The DNA sequence of selected parts of the inserted DNA is as predicted from the amino acid sequence of prelysozyme. The sequence data allows the unambiguous location of the coding region within lysozyme mRNA.
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
July 1977,
Nucleic acids research,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
July 1979,
Nucleic acids research,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
December 1979,
Proceedings of the National Academy of Sciences of the United States of America,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
November 1982,
Proceedings of the National Academy of Sciences of the United States of America,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
January 2002,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
January 1969,
Cold Spring Harbor symposia on quantitative biology,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
September 1979,
Proceedings of the National Academy of Sciences of the United States of America,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
September 1976,
Proceedings of the National Academy of Sciences of the United States of America,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
April 1981,
Die Naturwissenschaften,
A E Sippel, and
H Land, and
W Lindenmaier, and
M C Nguyen-Huu, and
T Wurtz, and
K N Timmis, and
K Giesecke, and
G Schütz
May 1978,
Proceedings of the National Academy of Sciences of the United States of America,
