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Nucleoside di- and triphosphates substituted for ascorbate in the thymine 7-hydroxylase reaction in studies carried out with purified preparations from Rhodotorula glutinis. The stimulations brought about by ascorbate and ATP were found not to be additive. Studies with analogues of ATP indicated that hydrolysis may not need to occur in order for the nucleotide effect to be expressed. The stoichiometry of the production of 5-hydroxymethyluracil and CO2 was not changed by the substitution of ATP for ascorbate. The 7-hydroxylase was found to have considerable thermal stability, and inactivation at 98 degrees C resulted in a parallel loss of the activities effected by ascorbate and ATP. This and the retention of the nucleotide effect upon purification suggest the effect is not mediated through another protein co-purified with the 7-hydroxylase.
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
September 1978,
The Journal of biological chemistry,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
May 2009,
Chemical research in toxicology,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
September 1983,
The Journal of biological chemistry,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
June 1970,
Doklady Akademii nauk SSSR,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
May 2005,
Biochimica et biophysica acta,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
July 1996,
Applied and environmental microbiology,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
January 1962,
Journal of bacteriology,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
January 1972,
Biokhimiia (Moscow, Russia),
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
January 1973,
Mikrobiologiia,
L M Wondrack, and
B J Warn, and
M D Saewert, and
M T Abbott
April 1976,
Biochemical and biophysical research communications,
