The paper presents results of experiments with fumigation of different concentrations of SO2 on Flamulina velutipes, Nematoloma fasciculare, and Pleurotus ostreatus. All the three concentrations of SO2, 84, 168, and 1.680 microgram/m3, had inhibitory effect on the growth of the fungi species. The inhibitory effect on the growth of the fungi species. The inhibitory effect increased with increasing SO2 concentration. All the three fungial species were prevented from growing by the concentration of 1.680 microgram/m3 SO2. Any changes in morphological structure of hyphae, were not observed.